human lung adenocarcinoma calu 3  (ATCC)

Journal: JID Innovations

Article Title: Human keratinocytes exhibit limited potential for SARS-CoV-2 infection despite ACE2 and mature cathepsin L expression

doi: 10.1016/j.xjidi.2025.100447

Figure Lengend Snippet: Quantification of ACE2 , dACE2 , TMPRSS2, NRP1, CTSL , BSG (CD147), DPP4 (CD26), AXL , KREMEN1 , and ASGR1 gene expression. mRNA levels normalized to GAPDH (2 -ΔCT ) in ( a ) NHDF, NHEKs, RHE, and human skin samples and in ( b ) Caco-2/TC-7, Calu-3, and HaCaT cell lines. Gene expression was quantified by RT-qPCR in triplicate. Data represent the mean ± SEM from ( a ) 3 to 14 independent experiments using biological samples from different individuals and ( b ) 3 or 4 independent experiments from cultured cells. NHDF, normal human dermal fibroblast; NHEK, normal human epidermal keratinocyte; RHE, reconstructed human epidermis.

Article Snippet: Human lung adenocarcinoma Calu-3 (Sterlab, Vallauris, France), human colon adenocarcinoma Caco-2/TC-7 (Sigma-Aldrich, St-Quentin-Fallavier, France), human epidermal keratinocyte cell line HaCaT (CLS, Köhln, Germany), and human promonocytic myeloid leukemia cell line U937 (ATCC, Manassas, VA) were maintained in complete MEM, IMDM, or DMEM medium (Gibco-Thermo Fisher Scientific, Courtaboeuf, France) supplemented with 10% fetal calf serum (Pierce, Thermo Fisher Scientific), penicillin (100 IU/ml), and streptomycin (100 μg/ml) (Gibco-Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% carbon dioxide.

Techniques: Gene Expression, Quantitative RT-PCR, Cell Culture

Journal: bioRxiv

Article Title: Antiviral drug synergy and mutational signatures in different epithelial cell models of RSV and hPIV infection

doi: 10.64898/2026.01.13.699296

Figure Lengend Snippet: A. Comparison of cell toxicity versus viral-GFP intensity (relative fluorescence units) at 7 days post-infection with RSVA or hPIV3 (both at MOI 0.02) with the epithelial cell lines VeroE6, Calu-3 or LLC-MK2 (n=3). Dashed box indicates cell lines chosen for subsequent antiviral assays. B-C. Representative images of the antiviral infection assay in 96-well format demonstrating dynamic range, here showing RSV infection of VeroE6 cells with and without single or dual-drug antiviral treatments. Cytopathology is visualised by endpoint crystal violet staining of adherent cells (B), whilst the fluorescence micrograph of same representative assay plate (C) demonstrates visualisation of GFP-tagged RSVA infection. D. Relationship between cell viability (quantified by crystal violet absorbance) and viral GFP fluorescence, as normalised against virus-only and mock controls. Representative dose response curves are modelled on data for remdesivir against RSVA. E-L. Dose response curves for monotherapy concentrations of remdesivir (E&I), favipiravir (F&J), EIDD-1931 (G&K), and ribavirin (H&L) tested against RSVA (E-H) or hPIV3 (I-L) infection. The viral inhibition % model is shown as the blue line, while cytotoxicity (as a % reduction of adherent cells) is indicated by the red line. The shaded box indicates drug concentrations with cytotoxicity exceeding 20%. (n=5 for RSVA, n=3-4 for hPIV3)

Article Snippet: Human lung adenocarcinoma cell line Calu-3 (HTB-55, ATCC) and rhesus monkey kidney cell line LLC-MK2 (CCL-7, Caltag-Medsystems Ltd) were maintained in DMEM supplemented with 10% fetal bovine serum (FBS, Thermo Fisher) and 1× penicillin/streptomycin.

Techniques: Comparison, Fluorescence, Infection, Staining, Virus, Inhibition